64 Citation Minion Virus
The minion sequencer coupled with our companion real time analysis tool the african swine fever fast analysis sequencing tool asf fast rapidly produced high quality high volume sequence data sufficient to detect and resolve the full asfv genome from cell culture isolates as well as whole blood samples from experimentally infected pigs. We summarize key technical features of the oxford nanopore minion the dominant platform currently available.
Nanopore Sequencing Review Of Potential Applications In
Citation minion virus
Article quick2017multiplexpm title multiplex pcr method for minion and illumina sequencing of zika and other virus genomes directly from clinical samples author joshua d. Ampseq accurately detected and genotyped both ibv lineages in 3 of 5 samples containing 2 ibv lineages. Nanopore dna strand sequencing has emerged as a competitive portable technology.Us to corroborate the inferences of our meth od. With the intention of comparing the potential utility of illumina and minion sequencing platforms within a. Taxonomic assignment of virus related reads produced by the minion sequencing technology.
Reads exceeding 150 kilobases have been achieved as have in field detection and analysis of clinical pathogens. Diagnosis and characterization of canine distemper virus through sequencing by minion nanopore technology. 24 may Citations multiplex pcr method for minion and illumina sequencing of zika and other virus genomes directly from clinical samples.
There is no effective vaccine to control african swine fever asf and therefore efficient disease control is dependent on early detection and diagnosis. Minion sequencing to genotype us strains of infectious laryngotracheitis virus. African swine fever virus asfv is the causative agent of a severe and highly contagious viral disease of pigs that poses serious economic consequences to the swine industry due to the high mortality rate and impact on international trade.
We then discuss pioneering applications executed by the genomics community. The ampseq method detected ibv in 13 of 14 ibv positive samples. We sequenced virus populati ons in parallel using both minion and illumina all owing.
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